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KMID : 1094719990040030189
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Biotechnology and Bioprocess Engineering
1999 Volume.4 No. 3 p.189 ~ p.194
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Sorbose mediated enhancement of cellulase biosynthesis inTrichoderma reesei
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Sethi Benu
Mishra Saroj
Bisaria Virendra S.
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Abstract
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Addition of L-sorbose, a non-metabolizable non-inducing ketohexose, toTrichoderma reesei cultures growing on cellobiose or Avicel-cellulose lead to increased cellulase activities. Addition of sorbose resulted in a 6-fold increase in cellodextrins (cellotriose, cellotetraose, cellopentaose) concentration on day 3 in cellobiose cultures and 1.3-fold increase in cellodextrins concentrations on day 4 in Avicel cellulose cultures. This increase in intracellular cellodextrins concentration matched closely with the increase in endoglucanase activity at these time points. Treatment of the cell-free extracts with cellulase preparation led to disappearance of the cellodextrins and increase of glucose. These observations suggested a more direct involvement of cellodextrins in cellulase induction process. The cellulases produced in sorbose-supplemented cellobiose medium hydrolyzed microcrystalline cellulose as effectively as the ones produced on Avicel cellulose medium.
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KEYWORD
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Trichoderma reesei, cellulase inducers, cellodextrins, sorbose
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